2009-05-27
15:45 at HCO G 7

Non-equilibrium fluctuations and mechanics of active gels and living cells

Fred Mackintosh

Vrije Universiteit, Amsterdam

Much like the bones in our bodies, the cytoskeleton consisting of filamentous proteins largely determines the mechanical response and stability of cells. Such important cellular processes as locomotion, cell division, and mechanosensing are largely governed by complex networks of cytoskeletal biopolymers and the associated proteins that cross-link these and/or generate forces within the network. In addition to their important role in cell mechanics, cytoskeletal biopolymers have also provided new insights and challenges for polymer physics and rheology. Biopolymer networks, for instance, exhibit strongly nonlinear rheology—in many cases stiffening by orders of magnitude when subject to shear strains of less than unity. In the cell, these polymer networks or gels are far from equilibrium in a way unique to biology: they are subject to active, non-thermal internal forces generated by molecular motors. We describe recent theoretical and experimental results on active networks in vitro that demonstrate significant non-equilibrium fluctuations due to motor activity [1,2]. Furthermore, such gels hold out the promise of active materials, whose stiffness can be controlled by enzymatic activity. We also show how fluctuations and dynamics of individual cytoskeletal filaments can be used to probe both mechanical properties and non-equilibrium activity in living cells [3,4]. 1. D Mizuno, C Tardin, CF Schmidt, FC MacKintosh, Science, 315:370 (2007). 2. FC MacKintosh and AJ Levine, Phys Rev Lett, 100:018104 (2008). 3. CP Brangwynne, FC MacKintosh, DA Weitz, PNAS, 104:16128 (2007). 4. CP Brangwynne, GH Koenderink, FC MacKintosh, DA Weitz, J Cell Biology, 183: 583-587 (2008).